Product Description
Mole Bioscience Reagent Extraction RNA / DNA
Umumnya penggunaan extraction kit RNA dan DNA dibagi menjadi :
- Boiling Method
- Magnetic Bead Method
- Spin Column
Viral DNA Extraction Kit – Boiling Method
Intended Use
This kit is intended for isolation of DNA of viruses, bacteria or human cells from human feces leach liquor, swabs, gastric mucosa and cervical cytological specimens. DNA purified by the kit is suitable for downstream applications such as PCR analysis, clone and sequence analysis. The kit is used in the detection of pathogenic microorganisms, forensic analysis, blood typing, and detection of genetic mutations.
Principle
This kit uses agents such as ethylenediaminetetraacetic acid (EDTA) and ethylphenyl polyethylene glycol(NP-40) to release the nucleic acid from the virus particles, bacteria or human cells after heating, while Chelex-100 resin binding to cations including Mg2+, which is an essential cofactor for DNase. Nucleic acids is purified after centrifugation.
Applicable sample types
Human feces leach liquor, swabs, gastric mucosa, cervical cytological specimens.
Packing specification
20 Tests / Bag, 24 Tests / Bag, 50 Tests / Bag, 100 Tests / Bag,25 Tests / Box( Pre-Aliquoted), 50 Tests / Box( Pre-Aliquoted)
Product Advantages
- High extraction efficiency, good nucleic acid yield, ensure the sensitivity of detection.
- Different extraction methods can be flexibly selected according to detection requirements.
Viral DNA / RNA Nucleic Acid Extraction Kit – Magnetic Bead Method
Intended Use
This kit can be used to quickly extract viral DNA and RNA from a variety of liquid samples such as human serum and plasma. The purified viral nucleic acid can be directly used in downstream analysis, such as PCR amplification, cloning, and sequence analysis. It is suitable for the diagnosis of pathogen, forensic analysis and genetic mutations detection. This kit can be used manually or automatically with applicable extractors to obtain DNA and RNA within 40 minutes.
Principle
Under the condition of Chaotropic salts (GH, GI, etc.) and an external magnetic field, the superparamagnetism of magnetic beads leads to the adsorption of nucleic acid. After properly washing, the nucleic acid is released in the elution buffer.
Applicable sample types
Serum, plasma, stool extract, swab lotion, etc.
Packing specification
32 Tests / Box, 64 Tests / Box
Product Advantages
- High extraction efficiency, good nucleic acid yield, ensure the sensitivity of detection.
- Different extraction methods can be flexibly selected according to detection requirements.
Viral DNA Extraction Kit – Spin Column
Intended Use
This kit can be used to quickly extract viral DNA and RNA from a variety of liquid samples such as human feces extract, swab lotion, etc. The purified viral nucleic acid can be directly used in downstream analysis, such as PCR amplification, cloning, sequence analysis, etc. It is suitable for the diagnosis of pathogen, forensic analysis and genetic mutations detection. This kit is manually used to extract DNA and RNA within 15-40 minutes.
Principle
This kit uses G lysis method to release the nucleic acid of pathogen in the sample. At the same time, the nucleic acid is adsorbed by the silica gel membrane, purified by washing and obtained by elution.
Applicable sample types
fecal extract, swab lotion, etc.
Packing specification
24 Tests/Box, 50 Tests/Box
Product Advantages
- High extraction efficiency, good nucleic acid yield, ensure the sensitivity of detection.
- Different extraction methods can be flexibly selected according to detection requirements.